Biophysics and the Challenges of Emerging Threats

Triplet O efficiently 2 2 quenches dye triplet states responsible for blinking. Singlet O reacts efficiently with organic dyes, 2 amino acids, and nucleobases [19, 20]. In the presence of saturating dissolved O , blinking of fluorescent dyes is sup- 2 pressed, but oxidative damage to dyes and biomolecules is rapid.