Techniques in Molecular Biology

Since the late 1950s and early 1960s, molecular biologists have learned to characterize, isolate, and manipulate the molecular components of cells and organisms. These components include DNA, the repository of genetic information; RNA, a close relative of DNA whose functions range from serving as a temporary working copy of DNA to actual structural and enzymatic functions as well as a functional and structural part of the translational apparatus; and proteins, the major structural and enzymatic type of molecule in cells. The use of molecular biology or molecular cell biology approaches in medicine is now called molecular medicine. The present book contains hands-on information on methods and protocols in a simplified manner which is very easy to learn and perform. Further, methods and protocols constitute a gold-standard reference for today's scientists who wish to develop and hone their molecular biology skills towards the discovery of new biological relationships. This book has been written to fulfill the requirements of graduate/postgraduate students and research scholars. <br><br> Contents: Regulation of Gene Expression Molecular Mechanisms Recent Developments in Stem Cell Technology Embryonic Stem Cell Stem Cell Research and Cloning Chemistry of Life Biochemistry of Genome