Isolation and characterization of hydrocarbonoclast bacteria

Microbiological analysis of hydrocarbon biodegrading bacteria isolated from leachate collected from the Ain T mouchent C.E.T. revealed that they belonged to the Pseudomonadaceae and Staphylococcaceae families. Biochemical tests revealed the presence of: Pseudomonas aeruginosa (Ps), Pseudomonas fluorescens (38 ) and Pseudomonas sp (98), Staphylococcus aureus (St), Staphylococcus xylosus (5), Staphylococcus hominis (78 ) and Staphylococcus warnerii (28). The latter were molecularly characterized by genomic DNA extraction and amplification of the 16S rDNA gene using universal primers. These bacteria were tested for the presence of the degradation gene catechol 2,3-dioxygenase (C230), by PCR amplification using primers specific to the latter. Of the seven strains studied, only Pseudomonas strains possessed this gene. The protein profile of the seven bacterial strains was then studied, enabling better characterization of hydrocarbonoclastic bacterial species...