Effect of lyophilization on microencapsulated probiotics

Probiotic microorganisms are very sensitive to environmental conditions and to the physical and chemical procedures necessary to formulate them in different pharmaceutical forms. The aim of this work is to find a suitable method of preservation of probiotic microorganisms once microencapsulated. For this purpose, different freeze-drying procedures are proposed and it is studied how they affect the survival of probiotics once microencapsulated and the characteristics of the microparticles.