Transketolases

Transketolase, Volume 724 in the Methods in Enzymology series, is devoted to the description of cutting-edge methods to its study in biochemistry. Chapters in this new release include Role of transketolase-like 1 in human cortical neurogenesis, Transketolase in metabolic diseases, autoimmune diseases and cancer, Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatments, Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitor, Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnostics, and much more.Additional sections cover Transketolases in apicomplexan parasites, Electrochemical screening for microbial transketolase inhibitors identification, Transketolases isolation from plants: Diversity of pathways, Transketolase-sulfoglycolytic pathway, Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp., Biophysical and structural analysis of transketolases, Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro study, Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisons, and much more. - Presents cutting-edge methodologies to study Transketolase, from cellular to molecular contexts- Details protocols described by selected experts that are easily applicable to other enzymes- Provides a compilation of methods for studying transketolases