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PCR Protocols in Molecular Toxicology
Tallenna

PCR Protocols in Molecular Toxicology

nidottu
, 1997
englanti
Sarja: Methods in Life Sciences - Toxicology Section

Molecular toxicology is an emerging discipline that utilizes molecular and cell biology to understand how drugs and chemicals result in their unwanted effects. PCR Protocols in Molecular Toxicology is a practical guide to the use of polymerase chain reaction (PCR) to help examine, on a molecular and cellular level, how toxic responses are manifested. It offers a basic understanding of PCR and its optimization, as well as describing specific, high-impact areas of molecular toxicology and recent advances. The following techniques are described in detail:

  • Quantitative reverse transcriptase PCR and methods to examine gene expression
  • Differential display cloning
  • Cloning and library screening by PCR
  • Genotype and polymorphism analysis of drug and toxicant metabolizing enzymes
  • Basic, non-PCR based molecular biology methods

    PCR Protocols in Molecular Toxicology will aid both novices and experienced PCR practitioners in using PCR to its fullest potential.
    • ISBN
    9780849333446
    Kieli
    englanti
    Paino
    376 grammaa
    Julkaisupäivä
    24.9.1997
    Kustantaja
    CRC Press Inc
    Sivumäärä
    252