Introducing specific mutations into the Escherichia coli chromosome using linear transformation

Diploma Thesis from the year 2003 in the subject Biology - Micro- and Molecular Biology, grade: 1 (A), University of Innsbruck (Institute for Microbiology), language: English, abstract: In 1885 Theodor Escherich described the gram negative bacterium Escherichia coli (E. coli)(Escherich T., Rev.1989). The gram negative rod belongs to the family of theenterobacteriaceae. It is a natural inhabitant of the human and animal intestine. E. coli canalso cause diseases like diarrhea, inflammation of the urinary tract or the gall bladder. Urinary tract infections (UTIs) are one of the most common reasons for antibiotic therapyworldwide (Burman and Olsson-Liljequist, 2001). People with UTIs suffer inflammation ofthe urinary tract, and frequently the kidneys. Two-thirds of patients with UTIs are women(Canbaz et al. 2002). This is related in part to the shortness of the urethra, which makescolonization of the bladder by bacteria more likely. The elderly and those who undergogenitourinary operations and catheterisation are also frequent sufferers of UTIs (Orenstein andWong, 1999). The leading causative agent of UTIs is E. coli (60-80 %), usually originatingfrom the patients own faecal flora, followed by Staphylococcus saprophyticus (10 %),Klebsiella sp., other Gram negative bacteria and enterococci (Burman and Olsson-Liljequist, 2001). The antibiotic class most frequently prescribed to treat UTIs in Western Europe andNorth America is the fluoroquinolones. Fluoroquinolones are synthetic antibiotics derivedfrom nalidixic acid. Resistance to the synthetic fluoroquinolone antibiotics is increasingamong the organisms that cause UTIs.