Enzyme Reprogramming

Enzyme Reprogramming, Volume 719 in the Methods in Enzymology series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Practical Considerations for Reaction Development with Vanadium-Dependent Haloperoxidases, Characterization and Engineering of thermostable PLP-dependent alpha-oxoamine synthases (AOSs); versatile C-C bond forming biocatalysts, Thiamine diphosphate Enzymes for Photobiocatalytic Enantioselective C(sp3)–H Acylation, Engineering a PLP-dependent Mannichase, Transferase engineering for SAM analog synthesis using combinatorial library design and high-throughput screening, Biaryl Coupling reactions catalyzed by Cytochrome P450s, and much more. Other chapters cover Engineering of a-ketoglutarate-dependent amino acid hydroxylases, Repurposing thiamine-dependent enzymes for radical biocatalysis, Directed evolution of nonheme Fe Enzymes for azidation, Directed evolution of P450 dearomatases for catalytic asymmetric radical dearomatization, Genetically encoded artificial photoenzymes for enantioselective [2+2] photocycloadditions, Unmasking the Reverse Activity of Old Yellow Enzymes for Selective Carbonyl Desaturation, and more.