Allergy Methods and Protocols

This book provides an extensive laboratory techniques for use by those involved in allergy research. Protocols cover everything form the preparation and storage of extracts, to the measurement of IgE and IgG, to the use of monoclonal antibodies. Also covered are important measuring techniques including those for airborne allergan exposure, allergens in dust reservoirs, and cross-reactivity. In keeping with the established format of the Methods in Molecular Medicine series, each chapter opens with a detailed introduction about a specific techniques which is followed by a comprehensive description of the equipment and reagents needed. Detailed step-by-step instructions describe how to perform each method and the Notes section provides invaluable information on trouble-shooting and optimization techniques that are drawn from the extensive experience of the authors.