Thiamine and thiamine-pyrophosphate (TPP) riboswitches

This volume of "Methods in Enzymology" series is devoted to the cofactor thiamine, also known as vitamin B1, and its phosphorylated derivatives. The biosynthesis of thiamine is discussed in terms of key enzymes and their regulation, the evolution of metabolic pathways, and its importance in plant and yeast metabolism. The importance of quantifying and distinguishing between phosphorylated and unphosphorylated thiamine in animal and human tissues using chromatographic, enzymatic, and nanopore methods is also discussed. In addition to these methods, thiamine riboswitches are a major focus of this book, covering everything from their identification in plants and the elucidation of their structure to their application in detection. This book presents state-of-the-art methods and detailed protocols intended for all researchers interested in thiamine metabolism or its detection.