Molecular typing of dandruff pathogens and evaluation of antifungal activity of plant extracts

Research Paper (undergraduate) from the year 2016 in the subject Biology - Micro- and Molecular Biology, , language: English, abstract: Dandruff is a common dermatological problem, causing dispersed flaking of the scalp and hair. Various intrinsic and environmental factors such as skin surface fungal colonization, individual susceptibility, are affecting the outbreak. The etiology of dandruff is not well understood. The study aims to verify the unequivocal understanding of the fungal relationship with dandruff by identification of filamentous fungi, Candida and survey of the bacterial companioning dandruff of hair samples and investigate the antifungal activity of water extracts of some medicinal plants in isolated fungi. A total of 280 hairs scalps samples, and hair swabs were collected from patients attenuated in Hilla hospitals and private clinics in the Babylon province of Iraq (n= 152 hair samples, n= 74 dandruff samples, n= 54 scalp swabs). Clinical samples were cultured on Sabouraud dextrose agar (SDA) medium with / without antibiotics based on stander cultured methods. Candida spp. was preliminary identified based on CHROMagar medium. Molecular typing of isolated yeasts via amplification ITS region and sequence analysis and multiple alignment was performed and constricted the phylogeny tree.