Overview of Blotting Techniques

There are 2 techniques that are commonly employed in research labs: gel electrophoresis and blotting. Following electrophoresis, separated nucleic acids and proteins can be transferred to a membrane where they are analyzed or detected. Depending on the molecule being analyzed, Southern blot refers to transfer and studying DNA, Northern blotting refers to RNA, Western blotting to proteins and eastern blotting for post-translational modifications. Given the importance of blotting in the lab, this book comes at an opportune time. The book discusses the protocols and applications of blotting-Southern, western, northern and eastern blotting. Case studies have also presented to help the reader gain insights into the practicality and applicability of blotting in today's world. The editors hope that this book will serve as a lab staple just like the aforementioned blotting techniques.