Exploring the mechanical features of biological cells, including their architecture and stability, this textbook is a pedagogical introduction to the interdisciplinary fields of cell mechanics and soft matter physics from both experimental and theoretical perspectives. This second edition has been greatly updated and expanded, with new chapters on complex filaments, the cell division cycle, the mechanisms of control and organization in the cell, and fluctuation phenomena. The textbook is now in full color which enhances the diagrams and allows the inclusion of new microscopy images. With around 280 end-of-chapter exercises exploring further applications, this textbook is ideal for advanced undergraduate and graduate students in physics and biomedical engineering. A website hosted by the author contains extra support material, diagrams and lecture notes, and is available at www.cambridge.org/Boal.